Detection of Salt Tolerant Gene (TaNIP) and Its Expression in Three Selected Wheat Genotypes Through Plant Breeding Programs Under Salinity Conditions

摘要

High soil salinity is a major abiotic stress in plant production worldwide. TaNIP gene was identified and cloned through the gene chip expression analysis of a salt- tolerant wheat mutant RH8706-49 under salt stress. Quantitative reverse transcription - PCR (Q-RT-PCR) was used to detect TaNIP salt tolerant gene and its expression in some selected wheat genotype for salt tolerance through plant breeding programs. The results of qualitative PCR Reaction- cDNA and Quantitative Real-Time PCR showed that the gene band appeared only in the selected genotypes with length 189bp, while this band absent in salt sensitive cultivar (Iraq) under salinity and non-salinity condition. Amount and expression of TaNIP gene to be enhanced under salinity condition only in the selected salt tolerant genotype, and they increased with increasing salt level. Great expression and amount of TaNIP gene was at high salinity level (20 ds/m). The selected salt tolerant genotype had proximately similar amount and expression of TaNIP gene under all salinity condition, while there had no amounts and expression of this gene in sensitive cultivar (Iraq) therefore according to this gene (TaNIP) there is improvement realized in these selected genotypes for salt tolerance through plant breeding programs.