In silico Approach in the Prediction and Analysis of the Three-dimensional Structure of Maleylacetate reductase: A Biodegrading Protein

摘要

With the advent of biological research in the field of environmental science, several microbes were found to act as the most important biodegradable molecules. Maleylacetate reductase being a member of oxidoreductase is mostly found in Pseudomonas species. This enzyme participates in three metabolic pathways: gamma-hexachlorocyclohexane degradation, benzoate degradation which are higher alkyl compounds involved in chemical pollutions in metropolitan cities. Determining its three-dimensional structure will lead to the structure function analysis and also might be helpful for designing receptors for the degradation of new chemical compounds. For this purpose, the amino acid sequence of the protein had been imported from UniProtKB database and the template was searched in BLASTp2.2.27. This template was then used for the prediction of the three dimensional structure of the protein by using SWISS-MODEL Workspace. The predicted model was then validated using SAVES server which gave an almost result for better prediction.