Genetic Transformation of Common Bean (Phaseolus vulgarisL.) with theGusColor Marker, theBarHerbicide Resistance, and the Barley (Hordeum vulgare)HVA1Drought Tolerance Genes

摘要

Five common bean (Phaseolus vulgarisL.) varieties including “Condor,” “Matterhorn,” “Sedona,” “Olathe,” and “Montcalm” were genetically transformed via the Biolistic bombardment of the apical shoot meristem primordium. Transgenes includedguscolor marker which visually confirmed transgenic events, thebarherbicide resistance selectable marker used forin vitroselection of transgenic cultures and which confirmed Liberty herbicide resistant plants, and the barley (Hordeum vulgare) late embryogenesis abundant protein (HVA1) which conferred drought tolerance with a corresponding increase in root length of transgenic plants. Research presented here might assist in production of betterP. vulgarisgermplasm.