Investigation of indole alkaloids in Bignonia capreolata

摘要

Purpose: Bignonia capreolata is a perennial semi-evergreenvine from the Eastern US that was used as medicine by theNative Americans but has since fallen out of use. The aim ofthis analysiswas to 1) verify the presence of the indole alkaloidreserpine in B. capreolata; and 2) if verified, generate an alkaloidrich fraction of B. capreolata to more accurately identifyand quantify reserpine.Methods: Initial confirmation of the presence of reserpinewas performed using high performance liquid chromatographyreferenced with an analytical standard of reserpine. Thealkaloid rich fraction was then tested via liquid chromatographyand mass spectrometry.Results: The presence of reserpine in B. capreolata has beenconfirmed and found to be 49 g per gram of leaf.Conclusion: This is the only known plant outside of theApocynacea plant family to contain this indole alkaloid.