Timely inhibitory circuit formation by Abl1 regulates innate olfactory behaviors in the mouse

摘要

Novel objective recognition test (NOR) was performed in week 15. Half of the brain hemisphere was collected for Western blotting for phosphorylated (p)-Tau protein level, and the other half was fixed for immunohistochemistry of the hippocampus for the apoptotic markers active caspase-3 and TUNEL. HFD consumption alone did not impair the performance during NOR, although p-Tau was reduced in the whole brain (p < 0.05), and Caspase 3 and TUNEL were increased in the hippocampus. In mice exposed to e-vapour, short-term memory function was significantly impaired, and was independent of the diet. E-vapour exposure significantly increased p-Tau levels, Caspase 3 and TUNEL positive cells in chow-fed mice but reduced p-Tau levels in HFD mice. In conclusion, long term e- vapour exposure led to impaired cognitive function independent of diet. Although HFD consumption alone increased p-Tau level and cell death, short-term memory function was not affected. There was no interaction between e-vapour exposure and HFD consumption on cognition in the current setting.