A novel PRRT2 pathogenic variant in a family with paroxysmal kinesigenic dyskinesia

摘要

Morphological properties of cholinergic neurons in the hippocampus of transgenic ChAT-cre mice Animal behavior is regulated by structural and connectional properties of neuronal networks. Therefore, identification and clas- sification of morphological features of cell assemblies is crucial to our understanding of perceptual ability. Despite evidence that the neuromodulator acetylcholine contributes to learning and mem- ory formation, anatomical descriptions of its expression remain incomplete. Hence, in an effort to advance our understanding of the neuronal substrates of cognition, the present study employed genetic manipulation, florescent microscopy, and improvements in digital storage capacity techniques to delineate the topographic expression of cholinergic neurons across wide areas of the mouse brain. Specifically, super resolution images of genetically encoded markers within cholinergic neurons were acquired from cerebral regions of Ai32XChAT-cre mice. Identification of cell body location confirmed the well-established loci of cholinergic cells in the stria- tum, basal forebrain, and brainstem. Surprisingly however, was the identification of a small group of cholinergic neurons in the dorsal subiculum. These neurons displayed dendritic spines and atypical cell body topologies. This study expands current models of cholin- ergic cell morphology and loci manifestation by demonstrating and describing the existence of a marginally understood network of cholinergic cells in an area of the brain known to regulate memory formation.