Assessment of Nature and Magnitude of Genetic Diversity Based on DNA Polymorphism with RAPD Technique in Traditional Glutinous Rice ( Oryza sativa L.) of Assam

摘要

Extent of genetic diversity based on DNA polymorphism with RAPD technique was studied at Assam Agricultural University, Jorhat during 2004-06 with seeds of 41 indigenous glutinous rice germplasm collected from two diverse agro-climatic zones prevailing in the Brahmaputra Valley and the Barak sub-basin of Northeast India. Ten random RAPD primers generated 214 fragments, out of which 209 were polymorphic (97.77%). With RAPD marker, the index of genetic similarities ranged from 0.078 to 0.623, 0.160 to 0.481 and 0.114 to 0.633 in overall, in Birain and in Bora-Chokuwa groups, respectively. Some of the RAPD fragments could be used to develop group specific markers (for example, OPK-14₂₀₀ in Bora-Chokuwa group and OPK-14₆₀₀ in Birain group).