OCIMUM SANCTUM EXTRACT COATING ON BIOMATERIAL SURFACES TO PREVENT BACTERIAL ADHESION AND BIOFILM GROWTH

摘要

Objective: The objective of this work is to evaluate the performance of OS extract as a coating on biomaterial surfaces in preventing bacterial adhesion and biofilm growth, as an effective measure to combat Biomaterial associated infections. Methods: Here, we have incorporated the extract from a medicinal plant as a coating to biomaterial surfaces in order to prevent bacterial adhesion and biofilm growth. To this end, Ocimum sanctum (OS) oil extract is coated on biomaterials (polymethyl methacrylate and polystyrene) and bacteria such as Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa were allowed to adhere and grow for 1 hr, 3 hrs and 24 hrs. Results: A significant reduction (p<0.01) in number of adherent bacteria on OS extract coated surfaces compared to bare surfaces was observed at all-time points. The zone of inhibition of OS extract was observed for all the three bacteria and maximum inhibition was observed for P. aeruginosa (30 mm diameter) compared to S. aureus (25 mm diameter) and E. coli (28 mm diameter). Conclusion: Thus, OS oil extract could be a promising coating for reduction of bacterial adhesion and biofilm formation.