ANGIOTENSIN I-CONVERTING ENZYME INHIBITORY ACTIVITY, TOTAL PHENOLIC AND FLAVONOID CONTENT OF EXTRACT AND FRACTION OF JAM FRUIT LEAVES (MUNTINGIA CALABURA L.)

摘要

Objective: Hypertension is one of the most common chronic diseases. Inhibitory activity of angiotensin I-converting enzyme (ACE) is effective on giving hypotensive effect. Jamfruit leaf ( Muntingia calabura L.) was reported to have an excellent hypotensive effect. This research was aimed to test in the manner of in vitro the inhibitory activity of ACE. Methods: This research used ACE kit-WST, total phenolic content, and total flavonoid from jam fruit leaf ethanol extract, hexane, ethyl acetate, and butanol fraction. Results: The result showed that Jamfruit leaf extract had ACE inhibitory activity and the most active fraction was ethyl acetate fraction. Inhibitory concentration 50% value of the most active fraction, ethyl acetate fraction was 0.63 μg/mL. Ethyl acetate fraction also provides most flavonoid and phenolic content with a value of 10.91 mg/g extract quercetin equivalent and 74.90 mg/g extract gallic acid equivalent. Conclusions: Ethyl acetate fraction of jam fruit leaf had most flavonoid, phenolic compound, and ACE inhibitory activity.