MULTICRITERIA OPTIMIZATION METHODOLOGY IN DEVELOPMENT OF HPLC METHOD FOR SIMULTANEOUS ESTIMATION OF INDAPAMIDE AND PERINDOPRIL IN BULK DRUG AND ITS COMBINED DOSAGE FORM

摘要

Multicriteria optimization methodology was applied for development of isocratic reversed-phased HPLC method for simultaneous determination of Indapamide and Perindopril. In the first stage of method development, pH value of the water phase, percentage of methanol, te mperature of the column and flow rate of the mobile phase were investigated using fractional factorial design. This work is concerned with application of simple, accurate, precise and highly selective reverse phase high performance liquid chromatographic (RP-HPLC) method for simultaneous estimation of Indapamide and Perindopril in combined dosage form. Chromatographic separation was achieved isocratically at 25°C ± 0.5°C on phase Inertsil ODS C8 [250 mm x 4.6mm] 5.m column with a mobile phase composed of Phosphate buffer with pH 2.5 and methanol in the ratio of 40:60 at flow rate of 0.5 ml/min. Detection is carried out using a UV-PDA detector at 215nm. The retention time of Perindopril at and Indapamide at 5.08 min and 6.91 min respectively. The correlation coefficients for all components are close to 1. The developed method was validated according to ICH guidelines and values of accuracy, precision and other statistical analysis were found to be in good accordance with the prescribed values. Thus the proposed method was successfully applied for simultaneous determination of Indapamide and Perindopril in routine analysis