NEURORESTORATIVE MULBERRIES POTENTIAL OF ALZHEIMER’S DISEASE IN ANIMAL MODEL

摘要

Objective: Alzheimer’s disease (AD) is one of the most common neurodegenerative disorders . The present study aimed to identify the potential neurorestorative of Morus alba and Morus rubra fruits extract on aluminum chloride (AlCl3)-induced AD rats. Methods: Each Morus species extract M. alba and M. rubra at a dose 300 mg/kg body weight (b.wt.) was orally administered, daily for 6 weeks post AlCl3 induction (100 mg/kg b.wt./day/6 weeks, orally). The polyphenols content was determined according to the colorimetric method of Folin–Ciocalteu using gallic acid as a standard. In addition, brain neurotransmitters, total protein content (TPC), and antioxidant biomarkers were determined. Histopathological assessment was performed on the brains of rats. Results: Administration of AlCl3 neurotoxicity induced a significant increment of brain acetylcholinesterase, and nitric oxide, while significant decrease in four monoamines (norepinephrine, epinephrine, 5-hydroxytryptamine serotonin, and dopamin), along with brain tissue ATPase, and TPC were determined. AlCl3-induced changes in peroxidative products, such as protein carbonyl, catalase, and superoxide dismutase activity. AlCl3 induction resulted in significant decrease in antioxidant enzymes, serum level of total antioxidant capacity, and caused histological alteration for brain tissue. Treatment of AD-rats with M. alba and M. rubra (300 mg/kg b.wt./day) causes modulation of most biochemical markers brain function and confirmed by brain histopathological study. Conclusion: Collectively, the present results indicated that M. rubra and M. alba exerts neuroprotection against AlCl3-induced brain pathology. To the best of our knowledge, none of the literature studies have demonstrated the effect of these Morus species against Al-neurotoxicity at biochemical and electron microscopic levels.