COMAPARTIVE STUDY OF ANTIBACTERIAL AND ANTI-PROLIFERATIVE POTENTIAL OF GREEN TEA FROM DIFFERENT GEOGRAPHICAL LOCATIONS IN INDIA

摘要

Objective: The aim of this study was to investigate the in vitro synergistic antibacterial effect of different solvent extracts of green tea (GT) with ampicillin and interpreting the anti-proliferative activity of purified GT catechins. Methods: The methanolic, acetone, and aqueous extracts of GT leaves consumed in Assam (AT), Himachal Pradesh (HP) and Uttarakhand regions (IP and PN) of North India were used in the study. The synergistic antibacterial properties of GT with ampicillin were determined against Pseudomonas aeruginosa microbial type culture collection (MTCC) 4306, Staphylococcus aureus MTCC 6908 and Escherichia coli MTCC 1698 using agar well diffusion assay. The purified catechins from different GT leaves were assessed for their anti-proliferative potential on C6 glioma cell lines using 3-(4, 5-dimethyl thiazol-2-yl)-5-diphenyltetrazolium bromide (MTT) assay. Results: The aqueous extract of different GT alone showed no inhibition against all bacterial strains used, whereas methanolic and acetone extracts revealed significant antibacterial activity (p<0.05). Combination of methanolic extracts of AT with ampicillin showed highest synergistic inhibition of 39±1.414 mm and 36±0.070 mm against S. aureus and P. aeruginosa respectively with an increase of 290±0.070% and 260±0.070% inhibition with respect to their individual effect. MTT assay depicted higher growth inhibition of 95.75±0.862-89.47±0.14% on treated glial cells. However, a significant difference in the inhibitory concentration (IC90) of the purified catechins from different GT leaves (p<0.05) was observed. Conclusion: GT leaves have potential anti-proliferative activity and synergistic antibacterial activity. Further in vivo and clinical studies are required to confirm its therapeutic efficacy.