FORMULATION AND EVALUATION OF HERBAL ANTIDEMENTIAL TABLETS

摘要

Herbs and their extracts/fractions are used for the treatment of different diseases and few as a memory enhancing agent. Different plants have been used as a memory enhancer in the folkloric medicine. Present study was undertaken to prepare a combined herbal formulation of the extracts of dried root powder of Plumbagozeylanica and powdered seeds of Silybummarianum for the treatment of dementia. HPTLC quantification characterization and identification of the herbal extracts of P. zeylanica and S. marianum, was performed using the standard marker compound, plumbagin and silibinin respectively of these drugs. Through the acute oral toxicity studies the dose of the extract in the formulation and a LD50 and ED50 profile of the drug combination were established. Design & development of tablet using two doses of the extract was done and evaluation was carried out on various standard parameters of formulation including accelerated stability tests, which was in turn found satisfactory and within the specified limits. Preliminary pharmacological screening of dementia was performed on formulated tablets using EPM (Elevated plus maze) and MWM (Morris water maze), two of the animal models of dementia. Here, Piracetam (400mg/kg p.o) a nootropic agent was employed as a standard drug. Sodium Nitrite (75mg/kg i.p) was used to induce amnesia in young experimental model, and which is comparable with that of the age related amnesia in old rats. The results indicate that administration of tablets produce significant dose dependant improvement of memory and were almost similar with that of standard drug Piracetam. In the present study antidemential activity of the combined herbal formulation of P. zeylanica and S. marianum and a few standardization parameters were tried to established, which can be further analysed and proceeded to prepare an effective antidemential formulation.