IDENTIFICATION AND CHARACTERIZATION OF POTENT ANTIMICROBIAL SECONDARY METABOLITE GENERATED FROM ENDOPHYTIC FUNGI ISOLATED FROM JAMBLANG PLANT ( Eugenia cumini L)

摘要

Objective : Colletotrichium capsici endophytic fungi isolated from Jamblang plant ( Eugenia cumini L). The secondary metabolites of this plant has the potent antibacterial efficacy as well as?diarrheal and anti-diabetic. This research focussed on isolation of the endophytic microbes from branches of Jamblang plants and their secondary metabolites. Method s: Isolation of endophytes were performed in PDA(potato dextrose agar) using direct seed plant. Endophytic fungi isolates with strongest antimicrobial activity against the bacteria Staphylococcus aureus, Escherichia coli were fermented in Potato Dextrose Yeast (PDY) to produce large scale of the metabolites. Supernatant was extracted with ethyl acetate solvent. Ethyl acetate extract fractionated by column chromatography (SiO3, n -hexane- ethyl acetate = 50:1 ~ 1:1) and obtained three fractions. Further, agar diffusion method was performed to assess their anti-microbial activity. Results : Antibacterial test results indicated?that fraction III?had the antibacterial activity Staphylococcus?aureus with inhibition zone?diameter of 10.7?mm but no observed antibacterial activity?against Escherichia?coli . Furthermore, identification by?GC-MS showed that compounds present in fraction III was mainly fatty acid and phenolic compounds. Conclusion: In conclusion, secondary metabolites isolated from Jamblang plants?branches contained predominantly fatty acid and phenol related compounds that could be responsible for its potent anti microbial activity. Keywords : Endophytic fungi, Jamblang ( Eugenia cumini L.), antimicrobial activity.