A NEW RAPID AND SIMPLE ANALYTICAL METHOD DEVELOPMENT AND VALIDATION OF ESTIMATION THE MYCOPHENOLATE IN DOSAGE FORM BY UPLC TECHNIQUE

摘要

A new rapid, reproducible and selective reverse phase UPLC method has been developed for the estimation of Mycophenolate in dosage form.It was resolved by using a mobile phase of Potassium dihydrogen phosphate: acetonitrile in the ratio 35:65 v/v at a flow rate of 0.2 ml/min. on UPLC system using UV - Visible detector at the wavelength of 228 nm. The column used was C18 (4.6 x 100mm, 3.5 mm, Make: XBridge).The linearity range was found to be 10-50 μg/ml. Quantification was achieved with UV detections at 216 nm based on peak area and absorbence. As per USP requirements system suitability studies were carried out and freshly prepared standard solutions are Mycophenolate The proposed new method is found to be economic, sensitive, precise, rapid and reproduc ible