A NEW, SIMPLE, SENSITIVE, ACCURATE & RAPID ANALYTICAL METHOD DEVELOPMENT & VALIDATION FOR SIMULTANEOUS ESTIMATION OF LAMIVUDINE, ABACAVIR & ZIDOVUDINE IN TABLET DOSAGE FORM BY USING UPLC

摘要

The present work was undertaken with the aim to develop and validate a rapid and consistent UPLC method in which the peaks will be appear with short period of time as per ICH Guidelines. The UPLC separation was achieved on a Symmetry C₁₈ (2.1 x 100mm, 1.7mm, Make: BEH) or equivalent in an Isocratic Mode. The mobile phase was composed of Phosphate Buffer (60%) [pH3.0] & Methanol (40%) [UPLC Grade] The flow rate was monitored at 0.25 ml per min. The wavelength was selected for the detection was 280 nm. The run time was 3min. The retention time found for the drugs Lamivudine, Abacavir and Zidovudine were 1.019 min., 1.271 min. & 1.617 min. respectively. The % recovery was found to be 98.0%- 99.0% for the drug Abacavir. The % recovery was found to be 98.0% – 99.6% for the drug Lamivudine. The % recovery was found to be 98.2% – 98.6% for the drug Zidovudine. The linearity was established in the range of 20 to 60ppm for the drug Abacavir & 10 to30ppm for the drug Lamivudine & 20 to60ppm for the drug Zidovudine. The LOD for the drugs Abacavir, Lamivudine and Zidovudine were found to be 0.002μg/ml, 0.003μg/ml, & 0.005μg/ml respectively. The LOQ for the drugs Abacavir, Lamivudine and Zidovudine were found to be 0.008μg/ml, 0.01μg/ml & 0.02μg/ml respectively. Overall the proposed method was found to be suitable, sensitive, reproducible, specific and accurate for the quantitative determination of the drug in tablet dosage form.