COMPREHENSIVE INVITRO EVALUATION OF PHARMACOLOGICAL ACTIVITIES OF SELECTED PLANT EXTRACTS AND GC-MS PROFILING OF FLACOURTIA JANGOMAS FLOWER EXTRACT

摘要

Objective: Continued exploration and bio-evaluation of plants hold good as there are an increasing demand and recognition of natural products in disease management. The aim of this study was to investigate the antioxidant, cytotoxic, and antiproliferative activity of methanolic extracts of different parts of six plants. Six plants - Couroupita guianensis, Flacourtia jangomas, Lucuma nervosa, Euphorbia milii, Acalypha hispida, and Hydnocarpus pentandra - were chosen for this study. Methods: The plant parts were extracted with methanol and screened for 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-azinobis-(3- ethylbenzothiazoline-6-sulfonic acid (ABTS) radical scavenging abilities. The cytotoxic activity of the extracts was investigated on SCC9 and Calu6 through MTT assay, and cell cycle was analyzed by flow cytometry to determine the antiproliferative activity of the extracts. The flower extract of F. jangomas was further subjected to gas chromatography-mass spectrometry (GC-MS) analysis for purification of the compounds of interest. A twoway ANOVA was done to estimate the effect of the extract between samples remembered at p<0.05 level. Results: Among all the plant extracts, the extract of F. jangomas (flower) showed significant antioxidant potential with IC50 values of 11.16±0.54 μg/ml and 12.34±0.37 μg/ml for DPPH and ABTS assays, respectively. GC-MS analysis of the extract revealed the presence of 21 phytocompounds. MTT assay revealed that this extract had promising cytotoxic activity against the two cancer cell lines, Calu6 and SCC9 with IC50 values of 43.57±0.04 μg/ml and 53.42±0.15 μg/ml, respectively. The extract treatment caused significant arrest in G2M phase of cell cycle. Conclusion: F. jangomas flower extract displayed significant antioxidant and antiproliferative activity and can be considered as a potential source of anticancer compounds.