NEUROIMMUNO MODULATION BY VESTIBULAR STIMULATION IN COLD WATER SWIMMING STRESS INDUCED WISTAR ALBINO RATS

摘要

Objectives: We hypothesized that vestibular stimulation prevents stress-induced suppression of immunity by inhibiting the stress axes and to test this hypothesis total leucocyte count, differential leucocyte count, platelet count, hemoglobin level, organ weight of spleen and liver were observed following vestibular stimulation in cold water swimming stress induced Wistar albino rats. Methods: Totally, 24 healthy, adult male albino rats of Wistar strain were used in the present study. Rats were forced to swim in the plastic tubs (height: 60 cm, diameter: 40 cm) containing cold water, maintained at 10°C. The middle ear cavity was irrigated with hot (40°C) or cold (15°C) water through a polyethylene tube for 15 days. Results: Data were analyzed by SPSS 20.0. Statistical tests used are two-way RM ANOVA and Bonferroni post-tests. Cold water vestibular stimulation was significantly prevented effects of stress on hematological parameters (p<0.05). Both cold and hot water vestibular stimulation effectively controlled stress induced changes on body weight and organ weights (p<0.05). Conclusion: Maximum effect of stress was observed on the 7th day in stress only group whereas this effect is minimized in cold water vestibular stimulation group and maintained in normal limits thereafter. We recommend further detailed study in this area is considered for further detailed study.