PCOS & SYMPATHETIC OUTCOME: ROLE OF THE CENTRAL AND PERIPHERAL NERVOUS SYSTEM IN OVARIAN FUNCTION OF RAT

摘要

Polycystic ovary syndrome (PCOS) is a complex, multifaceted, heterogeneous disorder, affecting 4% to 18% of reproductive-aged women and is associated with reproductive, metabolic and psychological dysfunction. Evidences from studies on women with PCOS and on an experimental rat PCO model suggest that the sympathetic regulatory drive to the ovary may be unbalanced (disturbance of ovarian noradrenaline homeostasis). The present study aimed the evaluation of sympathetic outflow in central and peripheral pathways in PCO rats. Our objectives in this study were (1) to estimate LC activity in rats with estradiol valerate (EV)-induced PCO; (2) to antagonize alpha adrenoceptor in systemic conditions with yohimbine and clonidine during modeling. Forty eight rats were divided into two groups: LC and drug groups. Every group subdivided in two groups: modeling and control. In modeling groups, eighteen rats were treated with estradiol valerate for induction of follicular cysts and remainder is sesame oil groups for control. Estradiol concentration was significantly augmented by the LC lesion in PCO rats (P<0.001), while LC lesion could not alter serum concentrations of LH and FSH, like yohimbine and clonidine. The morphological observations of ovaries of LC lesion rats showed follicles with hyperthecosis, but Yohimbine reduced the cysts, increased corpus lutea and developed follicles like clonidine. In this study the biochemical and histological findings show that simultaneous administration of yohimbine and clonidine with estradiol valerate can prevent induction of PCO modeling in rat.