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首页> 外文期刊>Asian Pacific Journal of Cancer Prevention >Comparison Between Two Detection Methods for HPV16,HPV18 and P16Ink4a Biomarkers in Diagnosis of Abnormal Cervical Cytology
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Comparison Between Two Detection Methods for HPV16,HPV18 and P16Ink4a Biomarkers in Diagnosis of Abnormal Cervical Cytology

机译:宫颈细胞学异常诊断中HPV16,HPV18和P16Ink4a生物标志物两种检测方法的比较

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摘要

Background: Cervical cancer is the second most common cancer among women in many populations. While the Pap smear is a well established screening test it suffers from both false-positive and false-negative results in diagnosis of cancers and precancerous states. In this study, immunocytochemistry of the P16 biomarker and HPV-PCR were compared for their diagnostic potential. Materials and methods: In the study, we obtained pairs of specimens from 45 women with cervical dysplasia. One sample was placed in a liquid-based solution, and processed for staining of sections with antibodies to P16. HPV-PCR was performed on the other and the results obtained were analyzed by T-test using SPSS v. 15. Results: Using HPV-PCR 71% of the samples were found to be infected with either HPV 16 or HPV 18, and the rate of infection did not have a statistically significant relationship with higher grades of dysplasia (p= 0.253). In contrast, with immunocytochemistry evaluation of P16, 64% of the specimens were positive, but the percentage of positive results significantly increased with higher grades of dysplasia (p= 0.0001). Conclusion: Employment of the P16 marker as an optional test might be preferable over HPV-PCR for cervical dysplasia in our geographical region.
机译:背景:宫颈癌是许多人群中女性中第二常见的癌症。虽然子宫颈抹片检查是一项完善的筛选测试,但在诊断癌症和癌前状态时会同时出现假阳性和假阴性结果。在这项研究中,比较了P16生物标志物的免疫细胞化学和HPV-PCR的诊断潜力。材料和方法:在这项研究中,我们从45位宫颈发育不良的女性中获得了成对的标本。将一个样品置于基于液体的溶液中,并进行处理以用针对P16的抗体对切片进行染色。另一方进行HPV-PCR,并使用SPSS v。15通过T检验分析获得的结果。结果:使用HPV-PCR,发现71%的样品感染了HPV 16或HPV 18,并且感染率与较高程度的异型增生没有统计学意义(p = 0.253)。相比之下,通过P16的免疫细胞化学评估,有64%的标本为阳性,但阳性结果的百分比随着不典型增生程度的升高而显着增加(p = 0.0001)。结论:在我们的地理区域中,使用P16标记作为可选测试可能比HPV-PCR更可取。

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