Association Study of Single-Nucleotide Polymorphisms of STAT2/STAT3/IFN-γ Genes in Cervical Cancer in Southern Chinese Han Women

摘要

Objective: Interferon-γ (IFN-γ) and signal transducers and activators of transcription (STATs) each play animportant role in carcinogenesis associated with viral infection. Cervical cancer is almost invariably associatedwith infection by human papillomavirus (HPV), and previous studies suggested that dysregulation of the signalpathway involved in IFN-γ and STATs is associated. Our objective was to evaluate the association of SNPsin STAT2, STAT3, and IFN-γ with cervical cancer susceptibility in Chinese Han women in Hunan province.Materials and Methods: Genomic DNA was extracted from peripheral blood samples of 234 cervical cancerpatients and 216 healthy female controls. STAT2 and STAT3 genotyping was performed using polymerase chainreaction-restriction enzyme (PCR-RE) analysis. IFN-γ genotyping was detected by PCR-amplification of specificallele (PASA). Results: For STAT2 rs2066807 polymorphisms, there was no significant difference of genotypedistribution (P=0.827) and allele frequencies (P=0.830, OR=1.09, 95% CI: 0.51-2.31) between cases and controls.For STAT3 rs957970 polymorphisms, there was no significant difference of genotype distribution (P=0.455)and allele frequencies (P=0.560, OR=0.92, 95% CI: 0.71-1.20) between cases and controls. For IFN-γ +874A/Tpolymorphisms, there was no significant difference of genotype distribution (P=0.652) and allele frequencies(P=0.527, OR=1.12, 95% CI: 0.79-1.59) between cases and controls. Conclusion: These results suggest thatpolymorphisms in STAT2, STAT3 and IFN-γ genes are not likely to be strong predictors of cervical cancer inHan women in southern China.