Sirt1 Gene Expression and Gastric Epithelial Cells Tumor Stage in Patients with Helicobacter pylori Infection

摘要

Introduction: The World Health Organization has categorized Helicobacter pylori as a carcinogen for gastriccancer, which causes human mortality worldwide. A number of studies have shown that H. pylori affects cell signalingin gastric epithelial cells and changes the expression of some proteins such as proinflammatory cytokines. Bacterialinfections may alter sirt1 and sirt2 genes expression in inflammatory tissues and cancer cells. In this study, sirt1 andsirt2 genes expression in gastric cancers was surveyed with reference to H. pylori status. Methods: Stomach biopsieswere collected from 50 gastric cancer patients, 25 H. pylori-positive and 25 H. pylori-negative as determined by theurea rapid test. Tumor grade was determined by a pathologist. After total RNA extraction from gastric cancer biopsysamples and cDNA synthesis, sirt1 and sirt2 genes expression levels were determined by Real Time PCR and ΔΔCTmethods. Results: There was no statistically significant link between H. pylori infection and sirt1 (P<0.899) and sirt2(P<0.169) genes expression in gastric epithelial cells. However, pathologic findings showed that there is a statisticallysignificant relationship between sirt1 gene expression and the tumor grade (P<0.024). Discussion: A statisticallysignificant association was found between sirt1 gene expression and tumor grade of gastric cancers that could be dueto effects on progression of cancer cells infected with H. pylori.