Revision of the European Lysiphlebus species (Hymenoptera: Braconidae: Aphidiinae) on the basis of COI and28SD2 molecular markers and morphology

摘要

Members of the genus Lysiphlebus Foerster are medium-sized endoparasitoid aphidiine wasps with over 30 species distributedworldwide. They attack numerous aphid hosts, and some species are important biological control agents. All European Lysiphlebus speciesare revised based on sequence information from the mitochondrial COI barcoding gene and the nuclear 28SD2 gene, morphological traits,and on information about their host specifcity. The taxonomic status of 13 European Lysiphlebus species is confrmed, while 11 are redescribed and illustrated. The following four Lysiphlebus species are synonymized: L. melandriicola Stary, 1961 [= L. fabarum (Marshall,1896)], L. hirtus Stary, 1985 (= L. confusus Tremblay & Eady, 1978), L. hispanus Stary, 1973 (= L. desertorum Stary, 1965), and L. safaviiStary, 1985 (= L. hirticornis Mackauer, 1960). Two new Lysiphlebus species are described: L. volkli sp.n. and L. brachycaudi sp.n. Ourresults, based on both COI and 28SD2, confrm the existence of three monophyletic species groups within the genus: (1) the ‘fabarum’group, which includes L. fabarum, L. cardui (Marshall, 1896), L. confusus, L. hirticornis, L. brachycaudi sp.n., and L. volkli sp.n.; (2)the ‘testaceipes’ group, which includes L. testaceipes (Cresson, 1880), L. orientalis Stary & Rakhshani, 2010, L. fritzmuelleri Mackauer,1960, L. balcanicus Stary, 1998, L. desertorum, [and probably L. dissolutus (Nees, 1811)]; and (3) the ‘alpinus’ group, consisting solely ofL. alpinus Stary, 1971. Geometric morphometrics of fore wing shape proved to be a powerful tool to delineate sibling species within the‘fabarum’ group. We were able to confrm for the frst time sexual lines of L. cardui and L. confusus. Furthermore, an additional potentiallyasexual Lysiphlebus taxon in Europe is revealed based on the discovery of an asexual line of L. volkli sp.n. in Iran. An illustrated key foridentifcation of the European Lysiphlebus species is provided.