Protective Role of Wheat Germ Oil against Hyperglycemia and Hyperlipidemia in Streptozotocin Induced Diabetic Rats

摘要

In this study, the possible protective effects of oral administration of wheat germ oil (WGO) at 2 doses (300 and 500 mg kg^?1 b.wt.) in normal, non-diabetic and streptozotocin (STZ) induced diabetic rats . Six groups of male Sprague Dawely rats were used; group 1: control negative, group 2: control negative treated with 300 mg kg^?1 b.wt., WGO, group 3: control negative treated with 500 mg kg^?1 b.wt., WGO, group 4: STZ diabetic rats , group 5: STZ diabetic rats treated with 300 mg kg^?1 b.wt., WGO and group 6: STZ diabetic rats treated with 500 mg kg^?1 b.wt., WGO. Blood and Serum analyses were carried out after 6 weeks (end of the experimental period) to determine the serum insulin hormone, fasting blood glucose levels (FBG), glycated hemoglobin percent (HbA1c), serum levels of Total Cholesterol (TC), triglycerides (TGs), low density lipoprotein cholesterol (LDL-C), very low density lipoprotein cholesterol (VLDL-C), high density lipoprotein cholesterol (HDL-C), TGs/HDL-C ratio and TC /HDL-C ratio. Liver tissue homogenate was used for evaluation of malondialdehyde (MDA) level. Microscopic examination of liver and pancreas, besides, estimation of caspase enzyme immunolabeling in both organs were made in all groups. Oral administration of 500 mg kg^?1 b.wt., WGO to non- diabetic rats significantly decreased HbA1c%, serum lipid profile , Atherogenic Index (AI), liver MDA and significantly (p>0.05) increased HDL-C level when compared to control negative group. In STZ induced diabetic rats , high dose of WGO was more effective than lower dose in correcting the significantly (p>0.05) elevated FBG, HbA1c, serum lipid profile , MDA levels and the significantly reduced levels of insulin hormone and HDL-C. The histopathological and immunohistochemical examination confirmed the biochemical results. About 500 mg kg^?1 b.wt., WGO has anti-hyperglycemic, anti-hyperlipidemic and has an antioxidant activity .