Effect of Semen Diluents, Dilution Rates and Storage Periods on Live and Abnormal Spermatozoa of Pearl Guinea Fowls

摘要

Background: Guinea fowls are neglected poultry with lower fertility rates compared to other avian species. Semen quality is one of the major determinants of fertility which deteriorates soon after collection. Therefore, the ability of different semen extenders in maintaining the livability and morphology of Guinea fowl spermatozoa under short term storage was studied. Materials and Methods: A total of 16 male Guinea fowls were housed in individual cages and was trained for semen collection by abdominal massage technique. Pooled semen samples were then diluted with two different semen extenders namely the beltsville poultry semen extender and instruments for veterinary medicine poultry semen preservation media each in the ratio 1:2 and 1:3. The diluted samples were then stored at 5°C and the percent spermatozoa livability and abnormality were assessed at 0, 3 and 6 h of storage. Results: Based on the study, the livability and morphologically normal spermatozoa were maintained above 80% with both the extenders until 6 h of storage. With respect to dilution rates, the semen in lower dilution had shown a higher percentage of live and morphologically normal spermatozoa irrespective of extenders and storage periods. The percent dead spermatozoa and abnormal spermatozoa significantly increased with increasing storage periods. Conclusion: Based on the results, it was concluded that the Guinea fowl semen may be extended with either of the diluents at lower dilution rates and the diluted semen must be inseminated within 3 h after collection which may have positive impact on fertility by artificial insemination.