Selection of Diluent for Short Term Preservation of Guinea Fowl Semen

摘要

This study was conducted to identify the suitable diluent for short-term preservation (0, 24 and 48 h) of Guinea fowl (pearl variety) semen. Semen samples were collected and diluted with normal saline, CARI, BPSE and Lake’s semen diluents and kept at6°C for storage. No significant difference was found in sperm motihty among the different diluents at 0 h (freshly ejaculated) storage period. A significant (p<0.05) reduction was found in sperm motility among all the diluents at 24 h storage period. Subsequently, there was no sperm motility was recorded in normal sahne diluted semen samples at 48 h storage period. While a reduction (p<0.05) in sperm motility was recorded in rest of the three semen diluents. The mean values for fertility with freshlyejaculated semen during 2-12 days of fertile period were recorded as 66.27±4.08, 78.90±3.08, 64.90±4.87 and 67.99±6.36% whereas, after 24 h storage of semen, fertility reduced drastically such as: 0, 41.97±7.27, 36.09±8.72 and 30.90±7.12% in normal saline, CARI, BPSE and Lake’s semen diluent, respectively. No fertility was noticed at 48 h storage in any semen diluent. Irrespective of the storage period and diluents, CARI semen diluent expressed better fertility than others in Guinea fowl.