Multiplex PCR and Detection of netB gene of Clostridium perfringens from Broilers with Necrotic Enteritis

摘要

Necrotic Enteritis (NE) is the most common and financially devastating bacterial disease in modern broiler flocks. A previously unknown pore forming toxin, which called new toxin Perfringens necrotic enteritis B-like toxin (NetB toxin) and the encoding gene, netB , which is produced by some Australian strains of Clostridium perfringens has recently been reported. This toxin was reported to be critical for the development of necrotic enteritis in chickens. To investigate the occurrence of the toxin gene ( netB ) in non-Australian C. perfringens strains, intestinal samples of fifty diseased broilers chickens and fifty apparently healthy broilers in Egypt were examined. The netB gene was found in some isolates while alpha toxin, which encoded by cpα gene was detected in all isolates from chickens. In contrast, none of the isolates carried the enterotoxin gene. The present study indicates the role of netB in the induction of necrotic enteritis in correlation with α-toxin while, no significant effect of enterotoxin of C. perfringens in the occurrence of this disease in broiler chickens.