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Iodine Disinfection of Sea Trout, Salmo Trutta (L.), Eggs and the Affect on Egg Surfaces

机译:海鳟,Salmo Trutta(L.),鸡蛋的碘消毒及其对鸡蛋表面的影响

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The aim of the research was to investigate the impact iodine solution disinfection had on Salmo trutta (L.) egg survival rates during incubation, and to determine the effect of the disinfection procedure on egg shells using scanning microscopy. The study groups were bathed in a Desamar K30 solution at a concentration of 100 ml per 10 dm-3 for 10 m once after the eggs had hardened and four times after the eyed stage. Egg samples for scanning analyses were collected on day 30 of incubation at the eyed stage after the first bath in the iodophor solution, and then at the end of incubation. Egg surface images were analyzed for the number of bacteria, the presence of hyphae, and the egg surface area covered with sediments. No statistically significant differences were noted in embryo survival rates in the groups that were disinfected. The highest number of bacteria was observed on egg surfaces which had not been disinfected prior to hatching. A significant amount of sediment was observed on the eggs during incubation. On day 90 of incubation, all of the egg surfaces were covered with sediments. Disinfection was not noted to have had a significant impact on the presence of hyphae. Iodophor preparations can be used for routine disinfection of trout eggs; however, other means of disinfection should be applied before the eyed egg stage.
机译:该研究的目的是研究碘溶液消毒在孵化过程中对杜鹃(Salmo trutta)卵存活率的影响,并使用扫描显微镜确定消毒程序对蛋壳的影响。在卵变硬后,将研究组在Desamar K30溶液中以10 ml 100毫升/ 10 dm-3的浓度沐浴10 m,然后在注视阶段后浸入四次。在孵化的第30天,在碘伏溶液中第一次洗完澡后,在眼部阶段收集鸡蛋样品进行扫描分析,然后在孵化结束时收集。分析了鸡蛋表面图像的细菌数量,菌丝的存在以及覆盖有沉积物的鸡蛋表面区域。在消毒组中,胚胎存活率没有统计学上的显着差异。在孵化前未消毒的鸡蛋表面观察到最多的细菌。孵化期间在卵上观察到大量的沉积物。在孵化的第90天,所有卵表面都被沉积物覆盖。没有发现消毒对菌丝的存在有重大影响。碘伏制剂可用于鳟鱼卵的常规消毒。但是,在带眼卵期之前应采用其他消毒方法。

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