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首页> 外文期刊>Archiva Zootechnica >Mesophilic and psychrotrophic aerobic sporulating microorganisms in raw cow’s milk
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Mesophilic and psychrotrophic aerobic sporulating microorganisms in raw cow’s milk

机译:生牛奶中的嗜温和精神营养性好氧孢子形成微生物

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The occurrence of mesophilic and psychrotrophic aerobic sporulating microorganisms (MPAS) in raw cow's milk (RCM) and its relations to microflora in milk was studied. 294 samples of raw cow's milk in 14 farms were taken during one year.Basis of the method for MPAS assessment is to inactivate the milk sample at the temperature 80–82°C during 30 minutes and incubation in cultivation dishes at 30°C for 3 days – mesophilic aerobic sporulates (MAS), and at 7°C for 10 days – psychrotrophic aerobic sporulates (PAS). Results of studied microbiological parameters characterize the studied milk as complying with requirements of the EU regulation 92/46 and standard STN 57 0529. Mesophilic and psychrotrophic aerobic sporeforming microorganisms count (MPAS) was within the span 2.5–340 CFU/ml. Average value of MPAS was 59.4 CFU/ml, and variation coefficient 93.1 %. Count up to 50 CFU/ml was in 55.4% samples, in 85% was the value not higher than 100, and in 3.1% samples was the count of MPAS higher than 200. MPAS do not show correlation with any of the studied microbiological parameters; marked influences of season were not observed either. On the basis of obtained results it is possible to support the proposal of initial limit 200 CFU/ml as a maximum for the introduction of MPAS standard parameter.MPAS count found in the same dishes at incubation for mesophilic and subsequently strictly psychrophilic microorganisms (MAS+SPAS) was on average 56.9 CFU/ml, it represents 95.8 % out of the number of sums of individual dishes at two temperatures. Correlation coefficient of these two types of results r=0.99 gives evidence of close dependence that is expressed by linear regression equation y=0.9773x. Use of two incubation temperatures one after another with identical set of dishes enables to exclude overestimation of results with sporulates able to grow at both incubation temperatures
机译:研究了生牛奶(RCM)中嗜温和精神营养性好氧孢子形成微生物(MPAS)的发生及其与菌群的关系。一年中抽取了14个农场的294份原奶样品。MPAS评估方法的基础是在80-82°C的温度下在30分钟内灭活牛奶样品,并在30°C的培养皿中保温3分钟。天–中温需氧孢子(MAS),在7°C下需10天–精神营养需氧孢子(PAS)。研究的微生物学参数结果表明所研究的牛奶符合EU法规92/46和标准STN 57 0529的要求。中温和精神营养性好氧芽孢形成微生物计数(MPAS)在2.5-340 CFU / ml之间。 MPAS的平均值为59.4 CFU / ml,变异系数为93.1%。在55.4%的样品中计数最高为50 CFU / ml,在85%的样品中数值不高于100,在3.1%的样品中的MPAS计数高于200。MPAS与所研究的任何微生物学参数均无相关性;也没有观察到明显的季节影响。根据获得的结果,有可能支持将MPAS标准参数引入最大值的初始上限200 CFU / ml的建议。在温育和随后严格嗜温的微生物(MAS + SPAS)平均为56.9 CFU / ml,在两个温度下占单个培养皿总数的95.8%。这两类结果的相关系数r = 0.99给出了由线性回归方程y = 0.9773x表示的紧密相关性的证据。在一组相同的培养皿中一个接一个地使用两个孵育温度可以排除高估结果的情况,因为孢子能够在两个孵育温度下生长

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