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Microsatellite primer development for the invasive perennial herb Gypsophila paniculata (Caryophyllaceae)

机译:入侵多年生草本满天星满天星(Caryophyllaceae)的微卫星引物开发

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Premise of the Study Gypsophila paniculata (baby's breath; Caryophyllaceae) is a herbaceous perennial that has invaded much of northern and western United States and Canada, outcompeting and crowding out native and endemic species. Microsatellite primers were developed to analyze the genetic structure of invasive populations. Methods and Results We identified 16 polymorphic nuclear microsatellite loci for G. paniculata out of 73 loci that successfully amplified from a primer library created using Illumina sequencing technology. Microsatellite primers were developed to amplify di‐, tri‐, and tetranucleotide repeats and tested in three invasive populations in Michigan. Conclusions These markers will be useful in characterizing the genetic structure of invasive populations throughout North America to aid targeted management efforts, and in native Eurasian populations to better understand invasion history. Five of these developed primers also amplified in G. elegans .
机译:研究的前提满天星(Gypsophila paniculata)(婴儿的呼吸;石竹科)是多年生草本植物,已侵袭美国北部和西部以及加拿大的大部分地区,与本地和特有物种竞争激烈。开发了微卫星引物来分析入侵种群的遗传结构。方法和结果我们从73个基因座中鉴定了16个多态核微卫星基因位点,该基因座已从使用Illumina测序技术创建的引物库中成功扩增。开发了微卫星引物来扩增二核苷酸,三核苷酸和四核苷酸重复序列,并在密歇根州的三个入侵人群中进行了测试。结论这些标记物将有助于表征整个北美的入侵种群的遗传结构,以帮助有针对性的管理工作,并有助于在欧亚大陆种群中更好地了解入侵历史。这些发达的引物中有五种也在线虫中扩增。

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