Improved RAPD Analysis of Canarium album (Lour.) Raeusch from Sichuan Province along Yangtze River in China

摘要

Aims: To build up the foundation of the classification and breeding of Canarium album cultivars in China. Methodology: The improved RAPD analysis was performed in nine samples by prolonging the ramp time from annealing to extension by using ten RAPD primers, out of twelve, which were selected randomly to analyze the genetic characteristics. Results: The polymorphism of the nine C. album was 86%, and the similarity coefficient ranged from 0.65 to 1.00. Among the similarity coefficients of the cultivars, the similarity coefficient of sample No. 2, 3 and 6 was highest (1.0), while the similarity coefficient of sample No. 5 and 7 was 0.98, which indicated that they are the same cultivars. The similarity coefficient of sample No. 9 and 4 was the lowest (0.65), which indicated that they are the different cultivars. Conclusion: Our study provides a theoretical basis for the breeding and classification of C. album in Sichuan Province along Yangtze River of China. Also this study suggests that the improved RAPD amplification could be an effective technique to analyze the polymorphism and genetic relationship in different organisms, especially, C. album.