Acetonitrile shortage: Use of isopropanol as an alternative elution system for ultra/high performance liquid chromatography

摘要

Acetonitrileisachoiceofsolventforalmostallchromatographicseparations.Inrecentyears,researchersaroundtheglobefacedacetonitrileshortagethataffectedroutineanalyticaloperations.Researcherstriedtocounterthisshortagebyapplyingmanyinnovativesolutions,includingusingultraperformanceliquidchromatography(UPLC)columnsthatareshorterandsmallerindiameterthantraditionalhighperformanceliquidchromatography(HPLC)columns,thussignificantlydecreasingthevolumeofeluentrequired.AlthoughutilizingUPLCinplaceofHPLCcanalleviatethesolventdemandtosomeextent,acetonitrileisgenerallythoughtofasthesolventofchoiceduetoitsversatility.Inthefollowingcommunication,wedescribeanalternativeeluentsystemthatusesisopropanolinplaceofacetonitrileasanorganicmodifierforroutinechromatographicseparations.WereportherethedevelopmentofanisopropanolbasedUPLCprotocolforG5PAMAMdendrimerbasedconjugatesthatwastransferredtosemi-preparativescaletopurifyfunctionalizedformsofthedendrimer(50mg)...