Sensitive determination of illegal drugs of clenbuterol and salbutamol in swine urine by capillary electrophoresis with on-line stacking based on the moving reaction boundary

摘要

The sensitive determination of clenbuterol (CLB) and salbutamol (SAL) in swine urine was developed by using capillary electrophoresis with a moving reaction boundary (MRB)-based stacking method. Under the optimum conditions, the MRB-based stacking procedure produced an improved concentration sensitivity of 70.5-fold for CLB and 24.7-fold for SAL. The improvement results in a limit of detection (LOD) of about 0.26 ng mLa?’1 and 0.96 ng mLa?’1 for CLB and SAL, respectively. The method has been successfully used for the analysis of CLB and SAL in swine urine, and the R.S.D. was less than 5.0%, the recoveries were in the range of 96.8a€“103.6% and the linear ranges of CLB and SAL were 0.003a€“10.0 ??g mLa?’1 and 0.01a€“20.0 ??g mLa?’1, respectively.