Bph14 gene determining brown-planthopper (Nilaparvata lugens Stal) resistance in rice varieties (Oryza sativa L.)

摘要

The brown planthopper (BPH) is one of the major insect pests of many rice areas in Vietnam [3]. Many researchers have reported that host plant resistance is the most effective way of controlling BPH, and thus breeding of insect resistance has taken priority in rice improvement programs [6]. In this study, we determined the present of bph14 gene in four rice varieties IRRI 352, BG 367-2, Sai Duong Kien An, Loc Nuoc. They were the BPH resistance rice varieties and their BPH resistant capacity were tested and supplied by Plant Resources Center, Science Institute of Agronomy, Hanoi, Vietnam. These rice varieties were cultivated and studied in Thua Thien Hue, Vietnam. From cds of bph14 gene sequence (http://www.ncbi.nlm.nih.gov, accession number: FJ941067.1) we have designed primers to identify of bph14 gene in these rice cultivars. Results showed that among these four rice varieties, bph14 gene was detected in Sai Duong Kien An and Loc Nuoc but it was not detected in IRRI 352 and BG 367-2.