Benzimidazole and imidazole fungicide analysis in grape and wine samples using a competitive enzyme-linked immunosorbent assay

摘要

This manuscript reports a quantitative pesticide residue method analysis employing an enzyme-linked immunosorbent assay (ELISA) for detecting carbendazim, imazalil and thiabendazole pesticide residues in vegetable samples. The results have been compared with those obtained from a liquid chromatography system coupled to a triple quadrupole mass spectrometer (LC-ESI-QqQ-MS). A validation study was undertaken to establish this method's performance characteristics on the 3 pesticide residues in two commodities (table grapes and wine). The carbendazim, imazalil and thiabendazole ELISA showed an IC50 reporting limit ranging from 0.5 to 13.8 ?μg kga?’1. The limit of quantification of the developed assay was 5 ?μg kga?’1 for both grape and wine matrices. The optimised assay was compared with a reference procedure based on liquid chromatography using samples acquired from various supermarkets, and a good correlation between both methods was found.