A plasmon resonance light scattering assay of glucose based on the formation of gold nanoparticles

摘要

A simple and label-free plasmon resonance light scattering (PRLS) assay for glucose was developed based on the formation of gold nanoparticles (AuNPs) from the redox between chlorauric acid (HAuCl4) and hydrogen peroxide (H2O2) in the presence of MES. It was found that the PRLS signals characterized at 550 nm were proportional to the content of H2O2, which could be produced in the process of biocatalytic reaction of glucose oxidase (GOx), and thus a RLS method of glucose was proposed with the linear range of 5.0 ?— 10a?’6 M to 1.0 ?— 10a?’4 M and the detection limit of 2.7 ?— 10a?’6 M.