The use of PAMAM dendrimers as a dynamic coating for cyclodextrin mediated enantioseparation of selected basic drugs

摘要

A dynamic coating using polyamidoamine (PAMAM) dendrimers was used to change the direction and magnitude of electroosmotic flow. The influence of PAMAM on electroosmotic mobility was studied in the concentration range from 0.005 to 0.100% (w/v) and at different electrolyte pH values from 2.5 to 8.5. PAMAM dendrimers, as an additive to the background electrolyte, were able to reverse the electroosmotic flow at a low concentration level (0.01% w/v). The presented dynamic coating was then applied to the enantioseparation of selected amines such as R,S- and S,R-tapentadol, R- and S-isoproterenol and R,S- and S,R-ephedrine with the use of 2-hydroxypropyl-?2-cyclodextrin as the chiral selector.