Method for obtaining conjugate of doxorubicin, trastuzumab and dendrimer PAMAM of 4th generation that has 64 amino groups on its surface and application of doxorubicin, trastuzumab and dendrimer PAMAM of 4th generation that has 64 amino groups on its surface for production of a drug intended for treatment of breast cancer

摘要

the object of the application is the way to receive doxorubicin conjugate, trastuzumab and dendrymeru pamam generation 4 having surface amino group and 64 application of doxorubicin.trastuzumab and dendrymeru pamam generation 4 having surface amino groups to produce 64 your medicine for therapy of breast cancer.process to obtain a conjugate of doxorubicin and trastuzumab and dendrymeru pamam generation 4 having amino group on the surface 64, is thatin the first stage, part of sulphurous acid cis - akonitowego undergo modification of amino groups of doxorubicin.next, in the presence of edc (ethyl n - (3-dimethylamino) propyl - n '- etylokarbodiimidu) is formed between the surface dendrymeru binding amide linkages, with the previously modified substrate.the reaction is carried out in pbs at room temperature.in the later stage of reaction, in order to connect to dendrymeru trastuzumab pamam uses adapter molecule n - sukcynoimidylo - 4 - (nmaleimidometylo) cykloheksylokarboksylan - (smcc) holding onits end functional group forming reaction with both tiolami and amines.in addition to the surface dendrymeru is tiolową - sh group, using trauta reagent (ethyl 2 - iminotiolanu).the modified dendrymerów pamam accompanied by monoclonal antibody previously sprzęgnięte hyphenated smcc.