Effects of Echinacea angustifolia Hell. extracts on total protein and peroxidase changing in Solanum lycopersicum L.

摘要

Echinacea angustifolia Hell. is an endemic, medicinal and economically important plant which is belonging to Asteraceae family. In this research, the effects of ethanolic and methanolic extracts of E. angustifolia (narrowleaved echinacea) on total protein and peroxidase [EC 1.11.1.7] levels changing on Solanum lycopersicum L. were determined. Echinacea powder extract were prepeared  as a stock solution by DMSO solvent as 0.01 g/mL and 0.03 g/mL concentrations. Extracts were applied to the S. lycopersicum L. plantlets by leaf spraying. 24 and 48 hours after applications, healthy leaf of 16 weeks old plantlets were harvested for protein and peroxidase analyses. Changing in  total protein and peroxidase levels were measured spectrophotometrically. Changing in both protein and peroxidase levels were occurred after echinacea applications acoording to concentrations and exposure time.  Amount of total protein changing after echinacea application in S. lycopersicum plantlets when compare with control group and 24 hours application. Protein levels were decreased 48 hours after 0.03g/mL echinacea application in DMSO, DMSO+methanol, DMSO+ethanol groups respectively as %43.16, %29.32 ve %27.26. Peroxidase activity changing in S. lycopersicum plantlets when compare with control group and 24 hours application. Peroxidase levels were increased 48 hours after 0.03g/mL echinacea application in DMSO, DMSO+methanol, DMSO+ethanol groups respectively as %43.16, %62.60, %38.20, %38.95. As a result of our research, echinacea extracts which is prepeared with two different concentrations and exposure times were stimulate the plant defense system as a plant activator as well.