Salt tolerance of endophytic Trichoderma koningiopsis YIM PH30002 and its volatile organic compounds (VOCs) allelopathic activity against phytopathogens associated with Panax notoginseng

摘要

Trichoderma spp. are widely used in the biological control of plant pathogens due to their high antagonistic and mycoparasitic potential. However, interaction among phytopathogens–Trichoderma–plant–soil is very complex and needs further investigation. In this study, we report the high salt-tolerant and mycoparasitic properties of endophytic T. koningiopsis YIM PH30002, isolated from the root of 2-year-old healthy Sanqi (Panax notoginseng), and its allelopathic antagonistic activity against phytopathogens associated with the host plant. Trichoderma koningiopsis YIM PH30002 exhibited significant inhibition of the growth of four host root-rot phytopathogens, Phoma herbarum, Fusarium flocciferum, Scytalidium lignicola, and Epicoccum nigrum, by covering the colony of phytopathogens, coiling and twisting the mycelium in a probable mechanism of mycoparasitism, producing volatile organic compounds (VOCs). In potato dextrose broth (PDB) culture medium, T. koningiopsis YIM PH30002 produced at least ten kinds of volatile substances which were identified as alkanes, monoterpenes and arenes, heterocycles, and aldehydes by GC-MS. The results indicate that YIM PH30002 can exert antagonistic actions by integrated ways to help its host defend diseases, and could be used as a promising candidate for the biological control of P. notoginseng root-rot disease.