Nanoparticles from Plant Saponins as Delivery System for Mucosal Influenza Vaccine

摘要

This article were studied nanoparticles assembled purified influenza virus hemagglutinin and neuraminidase antigens and low toxicity immunostimulating saponins Gg6 and Ah6, isolated from Kazakhstanian plants G.glabra and A.hippocastanum, in the animal vaccination/challenge experiments as delivery system for mucosal influenza subunit vaccine. Incorporation of influenza virus HA and NA antigens into the structure of nanoparticles significantly increased IgG, IgM and IgA antibody immune responses as well as production of IFN-gamma and IL-2 after single intranasal immunization. Mucosal immunization with nanoparticles possessed HA+NA antigens and saponins Gg6 or Ah6 resulted much better protection of chickens against challenge of highly pathogenic H7N1 avian influenza virus in comparison with immunization of whole virus inactivated vaccine or vaccine mixed with alum hydroxide adjuvant.