Effect of sex-sorted sperm on development and quality of in vitro-produced bovine embryos derived from ovum pick up oocytes

摘要

A combination of sex-sorted sperm and ovum pick up (OPU) -in vitro fertilization (IVF) techniques could improve the overall efficiency of the process and develop the commercial opportunities for sex-sorted sperm. Despite intrinsic differences which have been found between bovine oocytes collected post-mortem from slaughterhouse ovaries and those collected by OPU, only a few studies about IVF with sex-sorted sperm have used oocytes collected by OPU. In addition, to our knowledge, the effect of sex-sorted sperm on embryo development and quality using OPU-oocytes have been studied in buffalo(Bubalus bubalis)and in Bos indicus, but not in Bos taurus, therefore we aim to address this issue in this study. Oocytes were retrieved by OPU from mature dry cows and in vitro matured. In vitro fertilization was performed with sex-sorted or unsorted sperm