Phytochemical Screening, Antioxidant, Total Phenolic Contents, Cytotoxic and Phytotoxic Potential of the Methanolic Extract of Cornus macrophylla Wall Leaves

摘要

Phytochemical screening of the methanolic extract of Cornus macrophylla leaves (MECML) revealedvarious classes of phytochemicals. MECM demonstrated a concentration dependent antioxidant activity, inDPPH (1, 1-diphenyl-2-picrylhydrazyl) radical scavenging assay. Maximum activity (69.08%) was observed at100 μg/ml, with IC50, 57.87 μg/ml. Total Phenolic contents (TPC) were determined by Folin-Ciocalteu reagent(FCR) assay and was found 97.3±2.0 mg of gallic acid equivalent per gram of dry weight of the plant material.MECML exhibited profound cytotoxic potential (91%, 1000 μg/ml) against brine shrimp’s nauplii and wasdirectly proportional to the concentration (LC50,200μg/ml). Similarly MECML displayed the phytotoxic activityin a dose dependent manner (LD50, 24 μg/ml) and has shown remarkable phytotoxic potential, 95%±0.8 againstLamina minor at 1000 μg/ml. Thus on the basis of our findings one can conclude that the methanolic extractof C. macrophylla leaves is a rich source of antioxidants and natural herbicides.