Preparation of lamininidogen adsorbed urinary bladder decellularized materials via a mussel-inspired polydopamine coating for pelvic reconstruction

摘要

Pelvic organ prolapse (POP) is a serious health issue that affects many adult women. A common strategy for POP reconstruction is to use scaffold materials to reconstruct the prolapsed pelvic organ. However, the existing materials for pelvic reconstruction do not meet the clinical requirements for biocompatibility, mechanics and immunological rejection. To address these concerns, urinary bladder decellularized materials (UBDM) was selected due to their good strain-stress resistance. To enhance its biocompatibility, lamininidogen was used to modify the UBDM with a mussel-inspired polydopamine coating. We found that the biocompatibility and mechanical properties of lamininidogen-Dopamine-UBDM were significantly enhanced and that the degradation rate of lamininidogen-Dopamine-UBDM was markedly reduced. Moreover, the expression of CD31 in the lamininidogen-Dopamine-UBDM group was higher than that in the normal UBDM group. The lamininidogen-Dopamine-UBDM treatment mainly guided M2 type macrophages and led to an inflammatory response. These results indicate that lamininidogen adsorbed urinary bladder decellularized materials are promising for use in pelvic reconstruction.