首页> 外文期刊>American Journal of Plant Sciences >Developing Stable Cultivar through Microspore Mutagenesis in ×Brassicoraphanus koranhort, Inter-Generic Allopolyploid between Brassica rapa and Raphanus sativus
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Developing Stable Cultivar through Microspore Mutagenesis in ×Brassicoraphanus koranhort, Inter-Generic Allopolyploid between Brassica rapa and Raphanus sativus

机译:通过小孢子诱变开发稳定的品种,在Brassica rapa和Raphanus sativus之间的异源多倍体×Brassicoraphanus koranhort

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A stable progeny was developed through induced mutation, using microspore culture, of the hybrid (F1F1) produced by crossing a newly synthesized, unstable allopolyploid (F1) and a stable cultivar, BB#1(F1) in xBrassicoraphanus. An F1F1 plant was subjected to the induced mutation system established during production of BB#1. Morphological characteristics of the progeny such as color, and leaf number and length, differed from those of BB#1. The bolting time of the progeny in spring cropping was very late compared to BB#1, allowing it to be grown to an adult plant in spring. Genomic in situ hybridization analysis of pollen mother cells at prophase I identified 19 bivalents, 10 from Brassica rapa and 9 from Raphanus sativus. The glucoraphenin content was almost identical to that of BB#1. Two cultivars are available in the baemoochae crop now. These results indicate that induced mutation using microspore culture is a viable method of stabilizing intergeneric allopolyploids between B. rapa and R. sativus.
机译:通过使用小孢子培养物,通过在xBrassicoraphanus中杂交新合成的不稳定异源多倍体(F1)和稳定品种BB#1(F1)而产生的杂种(F1F1),通过诱变产生了稳定的后代。 F1F1植物经历了在BB#1生产过程中建立的诱导突变系统。后代的形态特征,例如颜色,叶片数目和长度,与BB#1不同。与BB#1相比,春季作物的子代抽time时间很晚,因此可以在春季长成。花粉母细胞在前期的基因组原位杂交分析确定了19个二价体,其中10个来自甘蓝型油菜,9个来自萝卜。糖皮质激素的含量几乎与BB#1相同。现在,在轮草作物中有两个品种。这些结果表明,使用小孢子培养物诱导的突变是稳定B. rapa和R. sativus之间属间同种多倍体的可行方法。

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