Plasma Cytokine Profiles as Predictive Biomarkers of HIV and Aids Progression among HIV Patients Attending Nakuru Provincial General Hospital, Kenya

摘要

Introduction: Cytokines are produced by many cell types, mostly cells of the immune system, and act on diverse targets, often the white blood cells. They play a central role in the pathogenesis of many diseases including Acquired Immunodeficiency Disease Syndrome (AIDS). They reflect the local or systemic inflammatory setting, and could serve as predictive biomarkers in Human Immunodeficiency Virus (HIV) disease progression. Objective: The aim of this study was to identify cytokines measureable in blood plasma in recently diagnosed HIV individuals before the commencement of antiretroviral therapy. Study Population: Eighty individuals, both males and females, were recruited for this study that comprised of forty newly diagnosed with HIV-1; twenty HIV negative individuals; and twenty HIV positive individuals currently on highly active antiretroviral therapy (HAART). Method: Cytokines were measured using multiplex cytokine immunoassay. Five types of cytokines were detected. Data analyses were performed using Graph Pad Prism 6. Independent sample T tests were used to compare the cytokine means while Spearman Rank tests were used to test for correlations. Statistical analysis were done using SPSS version 17. Results: The study showed significantly (p=<0.001) higher levels of IL-12p70, TNF, IL-10, IL-6 and IL-1β among the newly diagnosed HIV patients compared to those on highly antiretroviral therapy and HIV negative patients. Conclusion: Identification of plasma cytokines could be useful predictive biomarkers of HIV disease progression.