Determination of Antioxidant Compounds and In-Vitro Thrombolytic Activity of Borago Officinalis Leaves Growing Naturally in Kurdistan RegionIraq

摘要

ABSTRACT Phytochemical are compounds derived from natural source play important role in mankind health system with various physiological activities such as antioxidant and thrombolytic activity. Borago officinalis leaves have been assessed for quantity of antioxidant compounds comprise in estimation of total phenolic, total flavonoid and total tannin contents, and thrombolytic activity of hydrolacholic leaves extract at different concentration ranges. The hydroalcholic extract of leaves showed low phenolic contents about (26.45 ± 0.126 mcg GAEg DW) with approximately similar quantity of total flavonoid and total tannin contents about (212 ± 0.278 mcg QEg DW) and (216 ± 0.521 mcg GAEg DW), respectively. Leaf extract exhibited a dose dependent manner thrombolytic activity ranges between (8.346± 0.669 - 44.6697±1.1076) considered significant in comparison with control negative distilled water (p value 0.0001) and moderate activity in comparison with control positive streptokinase. Borago officinalis leaf was a good source for antioxidant compounds and open a venue for production of thrombolytic agents from the leaf with less side effects in comparison to the conventional drugs. Keywords: Borago officinalis, thrombolytic activity, total phenolic content, total flavonoid contents, total tannin contents.