Molecular detection of integron and antimicrobial resistance genes in multidrug resistant Salmonella isolated from poultry, calves and human in Beni-Suef governorate, Egypt

摘要

Antibiotic resistantSalmonellarepresent a public health threat worldwide. The current study was delineated to determineSalmonellaspp. prevalent in poultry droppings (N: 14 flocks), calves feces (N: 90) and human stool samples (N: 80) from live-stock contacts as well as poultry handler in Beni-Suef governorate, Egypt. Phenotypic and genotypic screening for the presence of antimicrobial resistance was applied. Isolation rates were 57.1%, 5.6% and 2.5% for poultry, calves and humans, respectively. S. Megherafelt. S. Kentucky, S. Amsterdam, S. Nigeria, S. Paratyphi: B2, S. Alamo, S. MadrasandS. Haifawere the predominant serovars in poultry flocks under investigation. The predominant serovar from calves’ samples wasS. KentuckywhileS. InfantisandS. Paratyphi: B2were detected in human samples. The results of antimicrobial disc diffusion revealed the presence of multiple drug resistance (MDR) in100% ofSalmonellaisolates. PCR confirmed the existence of class 1 integron and the following gene cassettes in the resistant strains,blaTEMandblaSHVfor β-lactams,aadA2for aminoglycosides (streptomycin),tetAandtetBfor tetracycline,sullfor Sulfamethoxazole,dfrAfor trimethoprim,floRfor chloramphenicol andmphAfor macrolides.