Determination of Iron Content in Different Hemoglobin Samples from Some Patients by UV-Visible Spectrophotometer

摘要

A validated, simple, accurate and precise spectrophotometric method has been proposed for the determination of iron in hemoglobin in human blood. The method is based on the reaction of iron with ammonium thiocyanate after the wet digestion of the blood samples with HNO₃ and H₂O₂. The optimum experimental conditions have been studied carefully. The absorption maxima was occurred at 475 nm and molar absorptivity of 0.03602 L mol^-1cm^-1. The linear regression line was obtained in the range of 0.5 - 60 μg/mL for iron content in hemoglobin. The detection limit and the limit of quantification were found to be 0.0583 and 0.1768 μg/mL respectively for the iron, and with R² value of 0.998. And recovery in range of 98.42 - 99.72%. Effects of pH, temperature, standing time and thiocyanate concentration on the determination of iron in human hemoglobin have been examined.