Characteristics of micro-propagated banana (Musa spp.) cultures stressed with NaCl and polyethylene glycol

摘要

The effect of NaCl and PEG was assessed on plant micro-propagation rate in banana (Musa?spp.) cv., Basrai. Well micro-propagated plantlets were cultured on four different stresses of NaCl and PEG-4000 including control level: MS2b?(MS0?+ 3.0 mg l-1?BAP), MS2c?(MS0?+ 100 mol m-3?NaCl), MS2d?(MS0?+ 5% PEG) and MS2e?(MS0?+ 100 mol m-3?NaCl + 5 % PEG) for 6-weeks. Efficiency of plant micro-propagation was reduced significantly among the stressed cultures. Similarly, photosynthetic pigments like chl?a?was decreased non-significantly but chl?b, chl?abwere decreased significantly. Total carotenoids were increased in the saline as well as PEG stressed cultures. Cell size of epidermis and aerenchyma was increased (p < 0.05), while parenchyma decreased. Proline and glycinebetain contents were increased (p < 0.05) in each stressed culture but were high in MS2?than in MS3?and MS4?cultures. Meanwhile, proteins, sugars, phenolics and nitrates were observed to be in the reversed (p < 0.05) phenomena. In conclusion, NaCl treatment was observed to be most toxic than the PEG or PEG with NaCl on the banana micro-propagation.